3THW
Human MutSbeta complexed with an IDL of 4 bases (Loop4) and ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.840, 116.130, 180.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.530 - 3.090 |
| R-factor | 0.2291 |
| Rwork | 0.226 |
| R-free | 0.28720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o8b |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.563 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.200 | 3.140 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.100 | 0.554 |
| Number of reflections | 41282 | |
| <I/σ(I)> | 7.5 | 1.1 |
| Completeness [%] | 99.0 | 98.5 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2M Ammonium Acetate, 20-25% PEG3350 (w/v) and 0.1M MES pH 6.5-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
