3THN
Crystal structure of Mre11 core with manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-03-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 138.859, 138.859, 123.102 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.058 - 2.811 |
R-factor | 0.1921 |
Rwork | 0.187 |
R-free | 0.24060 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.230 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.058 |
High resolution limit [Å] | 2.811 |
Number of reflections | 17314 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M Sodium sulfate, 0.1M Bis Tris propane, pH 6.5, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |