3TGQ
Crystal structure of unliganded HIV-1 clade B strain YU2 gp120 core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-07 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 222.970, 222.970, 86.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.270 - 3.400 |
| R-factor | 0.2805 |
| Rwork | 0.280 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rzk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.916 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.270 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.770 | 5.920 | 2.750 |
| Rmerge | 0.130 | 0.080 | 0.456 |
| Number of reflections | 39993 | ||
| <I/σ(I)> | 14.6 | 47.5 | 1.2 |
| Completeness [%] | 63.3 | 99.9 | 12 |
| Redundancy | 4.8 | 7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 16% PEG 3350, 0.1M CaCl2, 0.1M Tris-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.5 |
