3TG3
Crystal structure of the MAPK binding domain of MKP7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-08 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.497, 47.479, 64.512 |
| Unit cell angles | 91.18, 97.29, 96.81 |
Refinement procedure
| Resolution | 28.036 - 2.675 |
| R-factor | 0.2413 |
| Rwork | 0.239 |
| R-free | 0.28820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ouc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.194 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.036 | 2.720 |
| High resolution limit [Å] | 2.675 | 2.670 |
| Rmerge | 0.073 | 0.330 |
| Number of reflections | 11549 | |
| <I/σ(I)> | 8.6 | 2.54 |
| Completeness [%] | 95.0 | 93 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 100mM Bis-Tris, pH 6.0, 26% [w/v] polyethylene glycol 3350, 0.1M Ammonium Sulfate, VAPOR DIFFUSION, temperature 293K |
