3TF2
Crystal structure of the cap free human translation initiation factor eIF4E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.236, 56.739, 100.310 |
| Unit cell angles | 103.61, 91.36, 110.14 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | modified coordinates of pdb entry 1IPB |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.334 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.050 | 0.022 | 0.374 |
| Number of reflections | 44808 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 97.8 | 98.6 | 97 |
| Redundancy | 1.9 | 1.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 20% PEG-3350, 0.2 M diammonium citrate, vapor diffusion, temperature 289K |
