3TE6
Crystal Structure of the S. cerevisiae Sir3 AAA+ domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2009-09-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.590, 47.019, 127.852 |
| Unit cell angles | 90.00, 95.38, 90.00 |
Refinement procedure
| Resolution | 43.378 - 2.800 |
| R-factor | 0.2217 |
| Rwork | 0.219 |
| R-free | 0.27600 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.273 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.048 | 0.453 |
| Number of reflections | 26332 | |
| <I/σ(I)> | 9.31 | 2.4 |
| Completeness [%] | 93.8 | 99.9 |
| Redundancy | 1.67 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 2 M ammonium sulfate, 2% (w/v) PEG 400, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
