3TE6

Crystal Structure of the S. cerevisiae Sir3 AAA+ domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	298
Detector technology	CCD
Collection date	2009-09-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	89.590, 47.019, 127.852
Unit cell angles	90.00, 95.38, 90.00

Refinement procedure

Resolution	43.378 - 2.800
R-factor	0.2217
Rwork	0.219
R-free	0.27600
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.273
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.048	0.453
Number of reflections	26332
<I/σ(I)>	9.31	2.4
Completeness [%]	93.8	99.9
Redundancy	1.67	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	2 M ammonium sulfate, 2% (w/v) PEG 400, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K