3TDV
Structure of the GDP complex of wild-type aminoglycoside 2'-phosphotransferase-IIIa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-01 |
| Detector | RAYONIX MX-325 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.680, 54.650, 77.160 |
| Unit cell angles | 89.94, 103.83, 106.84 |
Refinement procedure
| Resolution | 38.452 - 2.200 |
| R-factor | 0.2086 |
| Rwork | 0.204 |
| R-free | 0.29110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.182 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.074 | 0.335 |
| Number of reflections | 30490 | |
| <I/σ(I)> | 9.6 | 2.51 |
| Completeness [%] | 95.0 | 94.3 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M lithium nitrate, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
