3TBW
CRYSTAL STRUCTURE OF THE MURINE CLASS I MAJOR HISTOCOMPATIBILITY COMPLEX H-2DB IN COMPLEX WITH THE LCMV-DERIVED GP33 ALTERED PEPTIDE ligand (A2G, V3P, Y4S)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | X-Flash XRF detector |
Collection date | 2009-09-09 |
Detector | Bruker AXS/Roentec X-Flash XRF detector |
Wavelength(s) | 0.91841 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.399, 124.277, 99.887 |
Unit cell angles | 90.00, 103.23, 90.00 |
Refinement procedure
Resolution | 47.482 - 2.150 |
R-factor | 0.2349 |
Rwork | 0.232 |
R-free | 0.28260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s7u |
RMSD bond length | 0.020 |
RMSD bond angle | 1.656 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.700 | 2.250 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.079 | 0.351 |
Number of reflections | 116037 | |
<I/σ(I)> | 11.8 | 2.5 |
Completeness [%] | 98.5 | 99.7 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Crystals were obtained in 1.6-1.8 M ammonium sulfate, 0.1 M Tris HCl pH 7.0-9.0 screening conditions. 4 ul of a 5mg/ml protein solution were mixed in a 4:2 ratio with the crystallization reservoir, VAPOR DIFFUSION, HANGING DROP, temperature 293K |