3TB8
Crystal structure of full-length myristoylated HIV-1 Nef
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-07-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.330, 89.330, 27.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.250 - 3.710 |
R-factor | 0.2334 |
Rwork | 0.231 |
R-free | 0.33660 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX (- Phaser) |
Refinement software | PHENIX (1.6.2_432 - Refine) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.250 |
High resolution limit [Å] | 3.710 |
Number of reflections | 2622 |
Completeness [%] | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 25% polyethyleneglycol 3350, lithium sulfate, HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |