3T8V
A bestatin-based chemical biology strategy reveals distinct roles for malaria M1- and M17-family aminopeptidases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-07 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.542, 108.821, 118.274 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.040 - 1.800 |
R-factor | 0.1622 |
Rwork | 0.160 |
R-free | 0.19760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.570 |
High resolution limit [Å] | 1.800 |
Number of reflections | 90083 |
Completeness [%] | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 22%(v/v) PEG8000, 10%(v/v) Glycerol, 0.1M Tris, 0.2M MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |