3T5O
Crystal Structure of human Complement Component C6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2009-11-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 146.810, 180.150, 60.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.057 - 2.869 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.27590 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.408 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 3.040 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Rmerge | 0.069 | 0.500 |
| Number of reflections | 35745 | |
| <I/σ(I)> | 6.4 | 1.5 |
| Completeness [%] | 94.9 | 60.5 |
| Redundancy | 8.3 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 284 | 5 mM MES, 89 mM Sodium Chloride, 0.01 mM Cadmium Chloride, pH 5.8, EVAPORATION, temperature 284K |
