3T5I
Structure of Fully modified farnesylated Rheb Peptide in complex with PDE6D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-31 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.750, 70.050, 71.230 |
Unit cell angles | 90.00, 81.55, 90.00 |
Refinement procedure
Resolution | 29.910 - 2.100 |
R-factor | 0.19137 |
Rwork | 0.189 |
R-free | 0.24309 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t5g |
RMSD bond length | 0.012 |
RMSD bond angle | 1.384 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 37401 | |
Completeness [%] | 99.6 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1 MES 6.5 and 30 % PEG 5000 MME , pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |