3T59

C76A/C455S mutant of mouse QSOX1 containing an interdomain disulfide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-06-16
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	42.698, 212.140, 148.476
Unit cell angles	90.00, 96.51, 90.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.2177
Rwork	0.218
R-free	0.27140
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.072	0.266
Number of reflections	64397
<I/σ(I)>	15.362	2.514
Completeness [%]	97.6	93.2
Redundancy	2.7	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	0.1M HEPES pH 7.0, 10% 1,5 pentadiol, 5% glycerol, VAPOR DIFFUSION, temperature 293K