3T45
Crystal structure of bacteriorhodopsin mutant A215T, a phototaxis signaling mutant at 3.0 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.452, 106.139, 124.845 |
| Unit cell angles | 90.00, 95.08, 90.00 |
Refinement procedure
| Resolution | 32.670 - 3.010 |
| R-factor | 0.23958 |
| Rwork | 0.237 |
| R-free | 0.28752 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.540 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.162 | 0.048 | 0.491 |
| Number of reflections | 15545 | ||
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 97.5 | 99.4 | 92.1 |
| Redundancy | 3.5 | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 301 | 3.0M MONOSODIUM PHOSPHATE pH 3.6, vapor diffusion, hanging drop, bicelles, temperature 301K, VAPOR DIFFUSION, HANGING DROP |
