3T36
Crystal structure of lytic transglycosylase MltE from Eschericha coli
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-07-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.8350 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.764, 94.925, 160.494 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.770 - 2.250 |
R-factor | 0.1923 |
Rwork | 0.189 |
R-free | 0.22350 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.174 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 24.770 |
High resolution limit [Å] | 2.250 |
Number of reflections | 56663 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M lithium sulfate, 15% PEG8000, 20 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |