3T1N

Structure of human MICROCEPHALIN (MCPH1) TANDEM BRCT domains in complex with a CDC27 phosphopeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-09-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	38.201, 46.506, 55.322
Unit cell angles	86.06, 70.69, 89.67

Refinement procedure

Resolution	28.710 - 2.600
R-factor	0.2322
Rwork	0.231
R-free	0.26230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t29
RMSD bond length	0.002
RMSD bond angle	0.669
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.710	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.100	0.340
Number of reflections	10161
<I/σ(I)>	5.6	2.2
Completeness [%]	92.2	94.9
Redundancy		2.29

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	295	30% PEG 3350, 0.3 M NACL, 0.1 M BIS-TRIS, PH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K