3T1E
The structure of the Newcastle disease virus hemagglutinin-neuraminidase (HN) ectodomain reveals a 4-helix bundle stalk
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 77.2 |
| Detector technology | CCD |
| Collection date | 2007-12-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97850 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 138.292, 138.292, 167.338 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.894 - 3.301 |
| R-factor | 0.2513 |
| Rwork | 0.248 |
| R-free | 0.31040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.001 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 105.409 |
| High resolution limit [Å] | 3.301 |
| Rmerge | 0.095 |
| Number of reflections | 25027 |
| Completeness [%] | 99.9 |
| Redundancy | 23.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 12% PEG 4000, 200 mM Li2SO4, 100 mM N-(2-Acetamido) Iminodiacetic Acid (ADA), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
