3T0P

Crystal structure of a Putative DNA polymerase III beta subunit (EUBREC_0002; ERE_29750) from Eubacterium rectale ATCC 33656 at 2.26 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-09
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97936
Spacegroup name	C 1 2 1
Unit cell lengths	127.760, 95.560, 84.010
Unit cell angles	90.00, 115.94, 90.00

Refinement procedure

Resolution	29.632 - 2.260
R-factor	0.1752
Rwork	0.173
R-free	0.20920
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2010)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.632	29.632	2.340
High resolution limit [Å]	2.260	4.850	2.260
Rmerge	0.049	0.020	0.578
Number of reflections	41878	7660	8062
<I/σ(I)>	11.62	32.4	1.8
Completeness [%]	93.8	89.7	95.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	36.00% 1,2-propanediol, 0.100M calcium acetate, 0.1M sodium acetate pH 5.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K