3T0O
Crystal Structure Analysis of Human RNase T2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-08 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9184,0.9810 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.200, 67.400, 47.900 |
| Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
| Resolution | 27.560 - 1.590 |
| R-factor | 0.15378 |
| Rwork | 0.152 |
| R-free | 0.19033 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dix |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.057 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER (/ SHELXE) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.310 | 1.690 |
| High resolution limit [Å] | 1.585 | 1.590 |
| Rmerge | 0.063 | 0.191 |
| Number of reflections | 26433 | |
| <I/σ(I)> | 171 | 5.04 |
| Completeness [%] | 98.0 | |
| Redundancy | 5.91 | 2.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 15% PEG3350, 0.2 M ammonium nitrate, macroseeding, no buffer, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
