3SYN
Crystal structure of FlhF in complex with its activator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.790, 63.360, 114.500 |
| Unit cell angles | 92.26, 100.96, 94.25 |
Refinement procedure
| Resolution | 63.091 - 3.063 |
| R-factor | 0.1838 |
| Rwork | 0.181 |
| R-free | 0.23880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2px3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.206 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.100 | 3.230 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Number of reflections | 25016 | |
| <I/σ(I)> | 8.5 | 5 |
| Completeness [%] | 95.2 | 93.6 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M Bis-Tris, 1.8 M Ammoniumsulfate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
