3SYC
Crystal structure of the G protein-gated inward rectifier K+ channel GIRK2 (Kir3.2) D228N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 86.488, 86.488, 179.627 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.040 - 3.410 |
| R-factor | 0.252 |
| Rwork | 0.251 |
| R-free | 0.27050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e4f |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.453 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.043 | 42.043 | 3.520 |
| High resolution limit [Å] | 3.400 | 7.320 | 3.400 |
| Rmerge | 0.092 | 0.048 | 0.931 |
| Number of reflections | 9865 | ||
| <I/σ(I)> | 11.7 | ||
| Completeness [%] | 99.5 | 96.7 | 99.9 |
| Redundancy | 5.6 | 5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293.15 | 50 mM sodium citrate, pH 6.0, 1 M sodium nitrate, 24-26% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
