3SYA
Crystal structure of the G protein-gated inward rectifier K+ channel GIRK2 (Kir3.2) in complex with sodium and PIP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 85.851, 85.851, 177.862 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.727 - 2.980 |
R-factor | 0.2419 |
Rwork | 0.240 |
R-free | 0.26920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2e4f |
RMSD bond length | 0.010 |
RMSD bond angle | 1.242 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.727 | 41.727 | 3.110 |
High resolution limit [Å] | 2.975 | 6.460 | 3.000 |
Rmerge | 0.108 | 0.048 | 0.827 |
Number of reflections | 13919 | ||
<I/σ(I)> | 10.2 | ||
Completeness [%] | 97.4 | 91.4 | 99.1 |
Redundancy | 5.8 | 5.5 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293.15 | 50 mM sodium citrate, pH 6.0, 1 M sodium chloride, 20% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |