3SXX
Hansenula polymorpha copper amine oxidase-1 in complex with Co(II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-08 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 103.887, 223.364, 103.969 |
Unit cell angles | 90.00, 95.61, 90.00 |
Refinement procedure
Resolution | 31.700 - 1.270 |
R-factor | 0.109 |
Rwork | 0.108 |
R-free | 0.13800 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | PDB ID 2OOV |
RMSD bond length | 0.019 |
RMSD bond angle | 1.890 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.320 |
High resolution limit [Å] | 1.270 | 1.270 |
Number of reflections | 1169541 | |
<I/σ(I)> | 23.5 | 3.4 |
Completeness [%] | 94.9 | 85.3 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 298 | 8.5% PEG 8000, 0.27 M potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |