3SV4
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in an open binary complex with dT as templating nucleobase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.388, 108.388, 90.467 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.933 - 1.990 |
| R-factor | 0.1807 |
| Rwork | 0.179 |
| R-free | 0.21610 |
| Starting model (for MR) | 4ktq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.770 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.7.1_743) |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.100 | 2.120 |
| High resolution limit [Å] | 1.990 | 1.990 |
| <I/σ(I)> | 13.79 | 2.06 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 9.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 18% PEG 8000, 0.2M MgFormate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
