3STA
Crystal structure of ClpP in tetradecameric form from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 168.572, 96.292, 192.614 |
Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.280 |
R-factor | 0.22641 |
Rwork | 0.224 |
R-free | 0.26946 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.993 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 155453 | |
Completeness [%] | 95.3 | 76.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 10% PEG 3000, 100mM cacodylate, 200mM magnesium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |