3SS7
Crystal structure of holo D-serine dehydratase from Escherichia coli at 1.55 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.774, 47.806, 75.193 |
| Unit cell angles | 90.00, 104.96, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.550 |
| R-factor | 0.161 |
| Rwork | 0.159 |
| R-free | 0.19390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.524 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.740 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 73947 | |
| <I/σ(I)> | 15.9 | 2.25 |
| Redundancy | 4.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.7 M sodium citrate, 0.1 M imidazole/maleate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
