3SPH
Inward rectifier potassium channel Kir2.2 I223L mutant in complex with PIP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-27 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | I 4 |
Unit cell lengths | 82.725, 82.725, 185.192 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.303 - 3.003 |
R-factor | 0.2186 |
Rwork | 0.217 |
R-free | 0.25370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3spg |
RMSD bond length | 0.012 |
RMSD bond angle | 0.977 |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.097 | 0.600 |
Number of reflections | 12263 | |
<I/σ(I)> | 27.4 | 2.7 |
Completeness [%] | 98.0 | 89.9 |
Redundancy | 6.3 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 12.1% PEG400, 0.5 M potassium chloride, 0.05 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |