3SPG
Inward rectifier potassium channel Kir2.2 R186A mutant in complex with PIP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | I 4 |
Unit cell lengths | 81.865, 81.865, 183.102 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.906 - 2.613 |
R-factor | 0.1969 |
Rwork | 0.195 |
R-free | 0.24110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jyc |
RMSD bond length | 0.009 |
RMSD bond angle | 1.015 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.059 | 0.355 |
Number of reflections | 17165 | |
<I/σ(I)> | 13.5 | 1.5 |
Completeness [%] | 88.0 | 71.1 |
Redundancy | 3.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 15.7% PEG400, 0.5 M potassium chloride, 0.05 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |