3SP8

Crystal structure of NK2 in complex with fractionated Heparin DP10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	172
Detector technology	PIXEL
Collection date	2011-01-29
Detector	PSI PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 61
Unit cell lengths	75.980, 75.980, 199.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.800 - 1.860
R-factor	0.1883
Rwork	0.187
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nh4
RMSD bond length	0.004
RMSD bond angle	0.883
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.800
High resolution limit [Å]	1.860	1.860
Number of reflections	54379
Completeness [%]	99.8	99.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	294	50mM MES pH=6.0, 30% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 21K, temperature 294K