3SOA
Full-length human CaMKII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 155.720, 155.720, 106.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.949 - 3.550 |
| R-factor | 0.2785 |
| Rwork | 0.273 |
| R-free | 0.32710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2VN9 AND 2UX0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.494 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.730 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Number of reflections | 8555 | |
| <I/σ(I)> | 1.7 | |
| Completeness [%] | 88.8 | 99.5 |
| Redundancy | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 288.15 | 1.2 M Ammonium Tartrate, 100 mM bistrispropane, pH 5.5, VAPOR DIFFUSION, temperature 288.15K |
