3SO4
Methionine-adenosyltransferase from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-21 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.492, 113.161, 220.848 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.180 |
| R-factor | 0.2306 |
| Rwork | 0.228 |
| R-free | 0.28390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.203 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.270 |
| High resolution limit [Å] | 3.180 | 7.880 | 3.200 |
| Rmerge | 0.171 | 0.040 | 0.716 |
| Number of reflections | 23472 | ||
| <I/σ(I)> | 4.8 | ||
| Completeness [%] | 92.2 | 97 | 56 |
| Redundancy | 3.8 | 4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.2 | 293 | protein buffer 25 mM HEPES pH 7.5, 500 mM NaCl; crystallization buffer 400 mM Ammonium Acetate, 100 mM BisTris pH 6.2, 33% PEG 3350, vapor diffusion, temperature 293K |
