3SMT
Crystal structure of human SET domain-containing protein3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-11-08 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 113.864, 113.864, 83.570 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.300 - 2.040 |
R-factor | 0.2095 |
Rwork | 0.208 |
R-free | 0.25910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.250 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.063 | 0.942 |
Number of reflections | 40192 | |
<I/σ(I)> | 40.8 | 2.76 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.9 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 20 % PEG4000, 0.2 M calcium Acetate, 0.1 M sodium cacodalyte, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |