3SLP
Crystal Structure of Lambda Exonuclease in Complex with a 12 BP Symmetric DNA Duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 65 |
| Unit cell lengths | 80.115, 80.115, 241.041 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.590 - 2.300 |
| R-factor | 0.21473 |
| Rwork | 0.211 |
| R-free | 0.28057 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1avq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.744 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.590 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.133 | 0.695 |
| Number of reflections | 32023 | |
| <I/σ(I)> | 14.9 | 2.3 |
| Completeness [%] | 87.1 | 77.2 |
| Redundancy | 7.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 295 | 22% PEG3350, 0.3 M sodium acetate, 0.1 M Tris, 5 mM calcium chloride, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
