3SLD
Structural characterization of a GII.4 2004 norovirus variant (TCH05) bound to A trisaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 244.537, 341.456, 124.772 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.655 - 2.679 |
| R-factor | 0.18343 |
| Rwork | 0.181 |
| R-free | 0.23029 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sjp |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.986 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.720 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.106 | 0.404 |
| Number of reflections | 143411 | |
| <I/σ(I)> | 12.68 | 2.35 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295.15 | 0.8M potassium sodium tartarate, 100mM Tris, 0.5% w/v PEG monomethyl ether 5000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
