3SJC

Crystal structure of S.cerevisiae Get3 in the semi-open state in complex with Get1 cytosolic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Collection date	2010-11-18
Wavelength(s)	0.873
Spacegroup name	P 1 21 1
Unit cell lengths	65.071, 112.243, 135.978
Unit cell angles	90.00, 103.82, 90.00

Refinement procedure

Resolution	47.436 - 3.200
R-factor	0.2119
Rwork	0.209
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.189
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.300	3.370
High resolution limit [Å]	3.200	3.200
Number of reflections	31634
<I/σ(I)>	7.3
Completeness [%]	100.0	100
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1 M LiCl, 21% PEG 6000, 0.1 M Tris/HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K