3SIQ
Crystal Structure of autoinhibited dIAP1-BIR1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 99.782, 99.782, 71.344 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.980 - 2.400 |
| R-factor | 0.14959 |
| Rwork | 0.146 |
| R-free | 0.21709 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sdz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.990 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 30880 |
| Completeness [%] | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 291 | 100mM Bis-Tris pH 5.4, 0.2M (NH4)2SO4, 3%( vol/vol) CH3OH, 22% (wt/vol) Polyethylene Glycol 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
