3SI0
Structure of glycosylated human glutaminyl cyclase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-26 |
| Detector | RAYONIX MX-225 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.408, 63.688, 77.159 |
| Unit cell angles | 90.00, 105.76, 90.00 |
Refinement procedure
| Resolution | 11.980 - 2.100 |
| R-factor | 0.20681 |
| Rwork | 0.204 |
| R-free | 0.26358 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2afm |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.996 |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 11.981 | 11.941 | 2.210 |
| High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
| Rmerge | 0.057 | 0.409 | |
| Number of reflections | 21435 | ||
| <I/σ(I)> | 7.1 | 9.7 | 1.9 |
| Completeness [%] | 96.0 | 75.7 | 96.3 |
| Redundancy | 3 | 3.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 100 mM imidazole, 30% v/v MPD, 11% w/v PEG4000, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
