3SHR
Crystal Structure of cGMP-dependent Protein Kinase Reveals Novel Site of Interchain Communication
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-12-23 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.156, 66.039, 81.550 |
| Unit cell angles | 90.00, 113.84, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rgs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.490 |
| Rmerge | 0.085 | 0.520 |
| Number of reflections | 32166 | |
| <I/σ(I)> | 18.7 | 1.9 |
| Completeness [%] | 97.9 | 93.5 |
| Redundancy | 4.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 2.2M (NH4)2SO4, 100mM Tris, 0.2% MPD at a protein concentration of 25-35 mg/mL , pH 8.0, VAPOR DIFFUSION, HANGING DROP |
