3SHI
Crystal structure of human MMP1 catalytic domain at 2.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-26 |
| Detector | OXFORD ONYX CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.694, 54.528, 94.905 |
| Unit cell angles | 90.00, 120.69, 90.00 |
Refinement procedure
| Resolution | 81.650 - 2.200 |
| R-factor | 0.21528 |
| Rwork | 0.209 |
| R-free | 0.27814 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 966c |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.021 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.650 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.066 | 0.490 |
| Number of reflections | 32923 | |
| <I/σ(I)> | 9.1 | 1.8 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 30% PEG8000, 0.1 M Tris-HCl, pH 8.5 |
