3SFU
crystal structure of murine norovirus RNA dependent RNA polymerase in complex with ribavirin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2010-04-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.952, 196.084, 109.206 |
| Unit cell angles | 90.00, 114.12, 90.00 |
Refinement procedure
| Resolution | 47.848 - 2.501 |
| R-factor | 0.1824 |
| Rwork | 0.181 |
| R-free | 0.22890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.141 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.093 |
| Number of reflections | 78946 |
| <I/σ(I)> | 21.41 |
| Completeness [%] | 100.0 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 277 | 1.26M ammonium sulphate, CHES (pH 9.5), 0.2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
