3SF4

Crystal structure of the complex between the conserved cell polarity proteins Inscuteable and LGN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2010-06-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9800
Spacegroup name	P 41 21 2
Unit cell lengths	124.815, 124.815, 233.851
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.720 - 2.600
R-factor	0.216
Rwork	0.216
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fo7 2wqh 1na0
RMSD bond length	0.006
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.096	0.422
Number of reflections	57538
<I/σ(I)>		6.17
Completeness [%]	100.0	100
Redundancy	12.1	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1M Tris HCl, 15% PEG 3350, 0.2M MgCL2, 4% 2,2,2-Trifluoroethanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K