3SF4
Crystal structure of the complex between the conserved cell polarity proteins Inscuteable and LGN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2010-06-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9800 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 124.815, 124.815, 233.851 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.096 | 0.422 |
Number of reflections | 57538 | |
<I/σ(I)> | 6.17 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.1 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris HCl, 15% PEG 3350, 0.2M MgCL2, 4% 2,2,2-Trifluoroethanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |