3SBO

Structure of E.coli GDH from native source

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-27
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.92001
Spacegroup name	P 21 21 21
Unit cell lengths	101.922, 151.617, 169.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.971 - 3.204
R-factor	0.2305
Rwork	0.229
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.424
Data reduction software	MOSFLM
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	43926
<I/σ(I)>	7.9	2.25
Completeness [%]	99.8	99.7
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	7.5	293	0.2M NaCl, 0.1 HEPES pH 7.5, 25% w/v PEG 3350, LIQUID DIFFUSION, temperature 293K