3SBB

Disulphide-mediated Tetramer of T4 Lysozyme R76C/R80C by Synthetic Symmetrization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 4 2 2
Unit cell lengths	83.988, 83.988, 58.923
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.236 - 1.434
R-factor	0.141
Rwork	0.139
R-free	0.17750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.222
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.000	80.000	1.480
High resolution limit [Å]	1.430	3.080	1.430
Rmerge	0.063	0.035	0.534
Number of reflections	38885
<I/σ(I)>	14.5
Completeness [%]	99.3	93.9	100
Redundancy	5.4	5.2	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	4.3M Sodium Chloride, 0.1M HEPES, pH 7.5, vapor diffusion, hanging drop, temperature 290K