3SBA

Zn-mediated Hexamer of T4 Lysozyme R76H/R80H by Synthetic Symmetrization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	55.470, 137.780, 146.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.765 - 2.750
R-factor	0.2248
Rwork	0.222
R-free	0.27790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.601
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.765		2.820
High resolution limit [Å]	2.750	12.300	2.750
Rmerge	0.219	0.179	0.490
Number of reflections	29680	473	3619
<I/σ(I)>	5.31	9.25	1.88
Completeness [%]	97.1	75.1	87.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	290	0.17M Ammonium Acetate, 0.085 Sodium Acetate Trihydrate, 25.5% PEG 4000, 15% Glycerol, pH 4.6, vapor diffusion, hanging drop, temperature 290K