3SAJ
Crystal Structure of glutamate receptor GluA1 Amino Terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-02-03 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.586, 94.421, 186.367 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.180 - 2.500 |
| R-factor | 0.2216 |
| Rwork | 0.218 |
| R-free | 0.28569 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.791 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.184 | 2.550 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.066 | 0.406 |
| Number of reflections | 57222 | |
| <I/σ(I)> | 10.3 | 3.5 |
| Completeness [%] | 99.7 | 97.5 |
| Redundancy | 5.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5 | 290 | 22% PEG3350, 0.2 M magnesium chloride, 0.1 M sodium acetate, pH 5.0, EVAPORATION, temperature 290K |
