3S9M
Complex between transferrin receptor 1 and transferrin with iron in the N-Lobe, cryocooled 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 104 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-03-29 |
| Detector | MAR345 |
| Wavelength(s) | 1.5417 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 232.187, 232.187, 168.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.000 - 3.320 |
| Rwork | 0.282 |
| R-free | 0.33150 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.284 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 17.000 | 17.000 | 3.440 |
| High resolution limit [Å] | 3.320 | 7.000 | 3.320 |
| Rmerge | 0.175 | 0.042 | 0.961 |
| Number of reflections | 65555 | ||
| <I/σ(I)> | 5.9 | ||
| Completeness [%] | 97.0 | 97.9 | 93.7 |
| Redundancy | 4.4 | 5.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 5% PEG 3350, 0.2 M MgCl2, 20% 1,2-propanediol, pH Hepes 7.5, vapor diffusion, hanging drop, temperature 293K |
