3S9L

Complex between transferrin receptor 1 and transferrin with iron in the N-Lobe, cryocooled 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 43 2 2
Unit cell lengths	231.676, 231.676, 168.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.220
Rwork	0.271
R-free	0.31430
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.330
High resolution limit [Å]	3.220	6.910	3.220
Rmerge	0.142	0.040	0.991
Number of reflections	74056
<I/σ(I)>	8.1
Completeness [%]	100.0	99.9	99.7
Redundancy	30.6	31.9	20.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	5% PEG 3350, 0.2 M MgCl2, 10% 1,2-propanediol, pH Hepes 7.5, vapor diffusion, hanging drop, temperature 293K