3S6B
Crystal structure of methionine aminopeptidase 1b from Plasmodium Falciparum, PF10_0150
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-05-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.868, 52.581, 63.506 |
| Unit cell angles | 90.00, 116.67, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.950 |
| R-factor | 0.1803 |
| Rwork | 0.178 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3h |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.413 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.150 | 1.900 |
| Rmerge | 0.069 | 0.033 | 0.637 |
| Number of reflections | 27052 | ||
| <I/σ(I)> | 11.7 | 1.81 | |
| Completeness [%] | 98.1 | 99.7 | 78.8 |
| Redundancy | 3.5 | 3.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 27% PEG3350, 0.2 M Ammonium Acetate, 0.1 M Tris, 2 mM TCEP, 20 % glycerol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
