3S62
Structure of Fab fragment of malaria transmission blocking antibody 2A8 against P. vivax P25 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 134.075, 177.819, 74.624 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 4.010 |
| R-factor | 0.26646 |
| Rwork | 0.264 |
| R-free | 0.31001 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z3g |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.663 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | |
| High resolution limit [Å] | 4.000 | 4.009 |
| Rmerge | 0.151 | |
| Number of reflections | 7444 | |
| <I/σ(I)> | 13.2 | 13.2 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 7.3 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M MES, 25% PEGMME550, 10mM ZnSO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
