3S5O
Crystal Structure of Human 4-hydroxy-2-oxoglutarate Aldolase Bound to Pyruvate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-07-29 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 141.230, 141.230, 108.388 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.648 - 1.970 |
R-factor | 0.1973 |
Rwork | 0.196 |
R-free | 0.21590 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.429 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.170 | 35.170 | 2.040 |
High resolution limit [Å] | 1.970 | 4.240 | 1.970 |
Rmerge | 0.050 | 0.029 | 0.354 |
Total number of observations | 66768 | 54992 | |
Number of reflections | 45370 | ||
<I/σ(I)> | 20.8 | 49.8 | 5.7 |
Completeness [%] | 99.9 | 99.2 | 100 |
Redundancy | 13.82 | 13.7 | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 3350, KSCN, Bis tris Propane, TCEP, Ethelyene glycol, pH 7.5, vapor diffusion, hanging drop, temperature 298K |